STUDIES ON THE DEXTRANSUCRASE OF LEUCONOSTOC DEXTRANICUM
نویسندگان
چکیده
منابع مشابه
Studies on Dextransucrase. I. Formation of Riboflavinylglucoside in Destran-producing Cultures of Leuconostoc Mesenteroides.
The dextran produced by this enzymic synthesis has been ascertained by many investigators to be composed largely of ƒ¿-1,6-glucopyranosidic linkage and some of ƒ¿-1 ,4 or ƒ¿-1,3-linkages. Dextransucrase, an enzyme which converts sucrose into dextran as well as fructose approximately equivalent to sucrose as was shown in equation (a), has been found in the cell-free culture fluids of various str...
متن کاملInduction and transcription studies of the dextransucrase gene in Leuconostoc mesenteroides NRRL B-512F.
Dextransucrase production by Leuconostoc mesenteroides NRRL B-512F in media containing carbon sources other than sucrose is reported for the first time. Dextransucrases were analyzed by gel electrophoresis and by an in situ activity assay. Their polymers and acceptor reaction products were also compared by (13)C nuclear magnetic resonance and high-performance liquid chromatography techniques, r...
متن کاملAcceptor reactions and mechanism of Leuconostoc mesenteroides B-512FM dextransucrase
14 INTRODUCTION 16 MATERIALS AND METHODS 19 Carbohydrates and reagents 19 Enzyme 19 Enzyme digest conditions 20 Digest conditions to obtain Hanes-Woolf plots 20 Digest conditions to obtain Michaelis plots 20 Determination of the amount of dextran synthesized 21 RESULTS AND DISCUSSION 22 REFERENCES 3 0 iii PAPER II INTERPRETATION OF DEXTRANSUCRASE INHIBITION AT HIGH SUCROSE CONCENTRATIONS 3 9 AB...
متن کاملLeuconostoc dextransucrase and dextran: production, properties and applications
This review covers the production, properties and applications of the biopolysaccharide dextran; this biopolymer can be produced via fermentation either with Leuconostoc mesenteroides strains and other lactic acid bacteria or with certain Gluconobacter oxydans strains. The former strains convert sucrose into dextran with the dextransucrase enzyme whereas the latter convert maltodextrins into de...
متن کاملThe nutritional requirements of Leuconostoc dextranicum for growth and dextran synthesis.
The recent literature contains numerous references to the use of partially hydrolyzed dextrans as plasma substitutes (Bull et al., 1949; Thorsen, 1949). Because of this interest, and as part of a general study of bacterial polysaccharide synthesis, the nutritional requirements of the major dextran forming organisms, Leuconostoc mesenteroides and L. dextranicum, are being investigated (Whiteside...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1953
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.65.2.136-140.1953